Immortalised CD40 deficient B Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Immortalised CD40 deficient B Cell Line
• Alternate name: Tumour Necrosis Factor Receptor Super family Member 5, CD4L Receptor, B Cell Surface Antigen CD4, TNFRSF5
• Research fields: Cell biology;Immunology
• Parental cell: B cells from CD40 deficient patient
• Organism: Human
• Disease: Hyper IgM Syndrome type 3
• Growth properties: Maintain at 3x105 to 7x105 cells per ml for optimal growth. Replenish growth medium twice per week
• Model: Immortalised Line
• Conditional: No
• Description: Can be used as antigen presenting cells. Hyper IgM syndromesÂ?‚ is a group ofÂ?‚ primary immune deficiency disordersÂ?‚ characterised by defectiveÂ?‚ CD40Â?‚ signalling;Â?‚ viaÂ?‚ B cellsÂ?‚ affectingÂ?‚ class switch recombinationÂ?‚ (CSR) andÂ?‚ somatic hyper mutation.Â?‚ ImmunoglobulinÂ?‚ (Ig) class switch recombination deficiencies are characterised by elevated serumÂ?‚ Immunoglobulin MÂ?‚ (IgM) levels and a considerable deficiency inÂ?‚ Immunoglobulins GÂ?‚ (IgG),Â?‚ AÂ?‚ (IgA) andÂ?‚ EÂ?‚ (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and IgA and normal or elevated IgM, leading to increased susceptibility to infections. Hyper IgM Syndrome type 3 is characterised by mutations of the CD40 gene. In this type, B cells cannot receive the signal from T cells to switch classes
• Production details: B Cells were isolated from PBMCs of CD40 deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.

Handling

• Format: Frozen
• Growth medium: RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50 ?„?žg/ml streptomycin
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice