Immortalised CD40 deficient B Cell Line

Can be used as antigen presenting cells. Hyper IgM syndromesÂ?‚ is a group ofÂ?‚ primary immune deficiency disordersÂ?‚ characterised by defectiveÂ?‚ CD40Â?‚ signalling;Â?‚ viaÂ?‚ B cellsÂ?‚ affectingÂ?‚ class switch recombinationÂ?‚ (CSR) andÂ?‚ somatic hyper mutation.Â?‚ ImmunoglobulinÂ?‚ (Ig) class switch recombination deficiencies are characterised by elevated serumÂ?‚ Immunoglobulin MÂ?‚ (IgM) levels and a considerable deficiency inÂ?‚ Immunoglobulins GÂ?‚ (IgG),Â?‚ AÂ?‚ (IgA) andÂ?‚ EÂ?‚ (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and […]

Contributors

Inventor
Pranab K Das
Cat. #: 154101
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Immunology
Organism: Human
Model: Immortalised Line
Growth properties: Maintain at 3×105 to 7×105 cells per ml for optimal growth. Replenish growth medium twice per week
Alternate name: Tumour Necrosis Factor Receptor Super family Member 5, CD4L Receptor, B Cell Surface Antigen CD4, TNFRSF5
Product description: Can be used as antigen presenting cells. Hyper IgM syndromesÂ?‚ is a group ofÂ?‚ primary immune deficiency disordersÂ?‚ characterised by defectiveÂ?‚ CD40Â?‚ signalling;Â?‚ viaÂ?‚ B cellsÂ?‚ affectingÂ?‚ class switch recombinationÂ?‚ (CSR) andÂ?‚ somatic hyper mutation.Â?‚ ImmunoglobulinÂ?‚ (Ig) class switch recombination deficiencies are characterised by elevated serumÂ?‚ Immunoglobulin MÂ?‚ (IgM) levels and a considerable deficiency inÂ?‚ Immunoglobulins GÂ?‚ (IgG),Â?‚ AÂ?‚ (IgA) andÂ?‚ EÂ?‚ (IgE). As a consequence, people with HIGM have decreased concentrations of serum IgG and IgA and normal or elevated IgM, leading to increased susceptibility to infections. Hyper IgM Syndrome type 3 is characterised by mutations of the CD40 gene. In this type, B cells cannot receive the signal from T cells to switch classes
Conditional: No
Production details: B Cells were isolated from PBMCs of CD40 deficient Hyper IgM Syndrome patients using standards methods. B cells were transformed with Epstein-Barr virus.
Parental cell line: B cells from CD40 deficient patient
disease: Hyper IgM Syndrome type 3
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 medium supplemented with 10% FCS, 10mM HEPES buffer, 50U/ml penicillin and 50 ?„?žg/ml streptomycin

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