Cat. #162302
IDHmut glioma stem-like NCH620 cell line
Cat. #: 162302
Organism: Human
Tissue: Brain
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Christel Herold-Mende
Institute: Heidelberg University
Primary Citation: Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: IDHmut glioma stem-like NCH620 cell line
- Cancer: Brain
- Organism: Human
- Tissue: Brain
- Growth properties: Mixed - adherent and suspension
- Description: Glioma stem-like cell line NCH620 expressing mutant isocitrate dehydrogenase (IDH), used to model low-grade gliomas or secondary glioblastomas
- Biosafety level: 2
Handling
- Growth medium: DMEM/Ham F-12 supplemented with 1% Penicillin/Streptomcyin, 1% 200mM Glutamine (preferably Glutamax), 10% BIT supplement (PeloBiotech), bFGF and EGF
- Temperature: 37°C
- Atmosphere: 5% CO2
- Storage conditions: FCS-free freezing medium (Synth-a-freeze medium)
References
- Trong et al. 2023. Cells. 12(8).
- Trong et al. 2020. Cells. 9(6).
- Trong et al. 2018. Int J Mol Sci. 19(10):2903. PMID: 30257451.
- Dettling et al. 2018. Clin Cancer Res. 24(12):2951-2962. PMID: 29563135.
- Kohanbash et al. 2017. J Clin Invest. 127(4):1425-1437. PMID: 28319047.
- Chiblak et al. 2016. Int J Radiat Oncol Biol Phys. 95(1):112-119. PMID: 26254681.
- Bunse et al. 2015. J Clin Invest. 125(2):593-606. PMID: 25555220.
- Campos et al. 2014. J Pathol. 234(1):23-33. PMID: 24756862.
- Dokic et al. 2012. Glia. 60(11):1785-800. PMID: 22951908.
