Cat. #161929
HT-29 Cell Line
Cat. #: 161929
Availability: 8-10 weeks
Tissue: Colon tissue
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jorgen Fogh
Institute: Memorial Sloan-Kettering Cancer Center (MSK)
Primary Citation: Fogh et al. 1977. Journal of the National Cancer Institute. 59.1.221. PMID: 327080.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HT-29 Cell Line
- Cancer: Adenocarcinoma;Digestive / Gastrointestinal cancer
- Gender: Female
- Tissue: Colon tissue
- Donor: This cell line was established in 1964 from the primary tumor of a 44-year-old Caucasian female with colorectal adenocarcinoma.
- Morphology: Epithelial
- Growth properties: Adherent
- Products or characteristics of interest: Karyotype: hypertriploid, modal number = 71; range = 68 to 72. Isoenzymes: AK-1, 1; ES-D, 1-2; G6PD, B; GLO-I, 2; PGM1, 1-2; PGM3, 2
- Description: HT-29 is a human colorectal adenocarcinoma cell line with epithelial morphology.
- Application: 3D cell culture, High-throughput screening, Toxicology, Cancer research
- Biosafety level: 1
Target Details
- Target: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5
Applications
- Application: 3D cell culture, High-throughput screening, Toxicology, Cancer research
Handling
- Growth medium: McCoy's 5a Medium Modified with fetal bovine serum to a final concentration of 10%.
- Temperature: 37° C
- Atmosphere: 5% CO2 in air
- Shipping conditions: Dry Ice
- Storage medium: Complete growth medium supplemented with 5% DMSO
- Str profiling: Amelogenin: X
References
- Nautiyal et al. 2011. Journal of molecular signaling. 6:7. PMID: 21774804.
- Cohen et al. 1999. Journal of cell science. 112.16.2657. PMID: 10413674.
