Histone deacetylase 1 (HDAC 1) knockout mouse embryonic stem cell line

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Histone deacetylases (HDAC) […]

Contributors

Inventor Institute
Shaun Cowley University of Leicester
Cat. #: 158383
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Developmental biology
Tissue: Embryo
Model: Knock-Out
Alternate name: RPD3L1
Product description: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Histone deacetylases (HDAC) 1 and 2 are highly similar enzymes that help regulate chromatin structure as the core catalytic components of corepressor complexes. Although tissue-specific deletion of HDAC1 and HDAC2 has demonstrated functional redundancy, germ-line deletion of HDAC1 in the mouse causes early embryonic lethality, whereas HDAC2 does not.
Conditional: Yes
Conditional description: Induction of Cre activity by addition of 4-hydroxy tamoxifen (OHT) to the growth media resulted in complete recombination of each allele and deletion of exon 2 (HDAC1Î?‚”2/Î?‚”2 or HDAC2 Î?‚”2/Î?‚”2) within 24 h
Production details: E14 ES cells, expressing a CreER fusion protein from the ROSA26 locus, were used to generate HDAC1Lox/Lox; CreER and HDAC2Lox/Lox; CreER cell lines by consecutive rounds of gene targeting.
Parental cell line: E14 ES cell line expressing an inducible Cre/Estrogen Receptor (CreER) construct from the endogenous ROSA26 locus
Disease: Embryo Lethality
Format: Frozen
Shipping conditions: Dry ice
Recommended controls: E14 ES parental line
References:

Dovey et al. 2010. Proc Natl Acad Sci U S A. 107(18):8242-7. PMID: 20404188.

Images

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Generation of HDAC1 and HDAC2 conditional knockout ES cell lines. (A) An E14 ES cell line constitutively expressing a Cre/Estrogen Receptor (CreER) fusion from the ROSA26 locus was used to generate homozygous conditional knockout alleles for HDAC1 and HDAC2. Both copies of exon 2 were flanked by LoxP sites

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