#152940

HeLa EGFP-Histone H2B Cell Line

Please Enter Your Email ID
Email ID

Cat. #152940

HeLa EGFP-Histone H2B Cell Line

Cat. #: 152940

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-5 days

Organism: Human

Tissue: Cervix

Model: Reporter

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Francis Barr

Institute: University of Liverpool

Tool Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HeLa EGFP-Histone H2B Cell Line
  • Alternate name: Histone H2B
  • Research fields: Cell biology;Genetics
  • Tool sub type: Continuous
  • Parental cell: HeLa
  • Organism: Human
  • Tissue: Cervix
  • Model: Reporter
  • Conditional: Yes
  • Description: The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
  • Biosafety level: 1
  • Recommended controls: HeLa parental line

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FBS, 5% CO2, 37?‚°C. Antibiotic resistance for selection of GFP positive cells: 4 ?g/ml Blasticidine, expression of GFP is quite stable but selecting at least every two passages is recommended.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line

References

  • Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
  • Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.