HeLa EGFP-Histone H2B Cell Line

The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.

Contributors

Inventor Institute
Francis Barr University of Liverpool
Cat. #: 152940
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Genetics
Organism: Human
Tissue: Cervix
Model: Reporter
Alternate name: Histone H2B
Product description: The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
Conditional: Yes
Parental cell line: HeLa
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FBS, 5% CO2, 37?‚°C. Antibiotic resistance for selection of GFP positive cells: 4 ?g/ml Blasticidine, expression of GFP is quite stable but selecting at least every two passages is recommended.
Recommended controls: HeLa parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.

Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.

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