HEK293T CAGA12-Luc/TK Renilla cell line

The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.

Contributors

Inventor Institute
Caroline Hill The Francis Crick Institute
Cat. #: 156438
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Cell signaling and signal transduction;Developmental biology
Organism: Human
Tissue: Kidney
Model: Reporter
Product description: The human embryo kidney cell line HEK293T modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
Production details: Made by transfecting the human embryo kidney cell line HEK293T with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.
Additional notes: Unpublished
Parental cell line: HEK293T cell line
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
Recommended controls: HEK293 parental line
Mycoplasma free: Yes
Biosafety level: 1
References:

Gori et al. 2021. eLife 8,10:e63545. PMID: 33416497

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