#156438

HEK293T CAGA12-Luc/TK Renilla cell line

Cat. #156438

HEK293T CAGA12-Luc/TK Renilla cell line

Cat. #: 156438

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Kidney

Model: Reporter

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Caroline Hill

Institute: The Francis Crick Institute

Tool Details
Target Details
Handling
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HEK293T CAGA12-Luc/TK Renilla cell line
  • Tool sub type: Continuous
  • Parental cell: HEK293T cell line
  • Organism: Human
  • Tissue: Kidney
  • Model: Reporter
  • Conditional description: In the presence of activin and TGF-beta signalling luciferase is expressed.
  • Description: The human embryo kidney cell line HEK293T modified for use as a reporter cell line for Activin and TGF-beta signalling. The cell line stably expresses CAGA12-Luc, a Smad3/Smad4-dependent reporter containing 12 copies of the CAGAC sites (AGAC motif underlined) from the PAI-1 promoter. This is a Smad3/Smad4-dependent reporter, although it also binds complexes of Smad2 exon 3 with Smad4. The cell line also contains a Renilla reporter driven by the thymidine kinase (TK) promoter to act as an internal control.
  • Production details: Made by transfecting the human embryo kidney cell line HEK293T with CAGA12-Luc with pRetroSuper for puromycin resistance and then TK Renilla with pCMV-Bsd for blasticidin resistance.
  • Biosafety level: 1
  • Additional notes: Unpublished
  • Recommended controls: HEK293 parental line

Target Details

  • Target: Activin and transforming growth factor beta (TGF-beta)¬†signalling

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal calf serum (FCS)
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

Related Tools

  • Related tools: MDA-MB-231 CAGA12-Luc/TK Renilla cell line

References

  • Gori et al. 2021. eLife 8,10:e63545. PMID: 33416497