Cat. #162181
ENO 1 F423A M2 HAP1 cell line
Cat. #: 162181
Organism: Human
Tissue: Bone marrow
Disease: Membranous glomerulonephritis
Model: Genetically modified cell line; knock-in
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Marit Otterlei
Institute: Norwegian University of Science and Technology
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: ENO 1 F423A M2 HAP1 cell line
- Parental cell: HAP1
- Organism: Human
- Gender: Male
- Tissue: Bone marrow
- Donor: 40 years old
- Disease: Membranous glomerulonephritis
- Morphology: Fibroblast-like
- Growth properties: Adherent
- Model: Genetically modified cell line; knock-in
- Model description: mutated PCNA interacting domain APIM (F423A) in the ENO1 gene
- Crispr: Yes
- Products or characteristics of interest: near-haploid cell line containing half of the usual number of chromosomes
- Description: Chronic myelogenous leukaemia cell line with mutated PCNA interacting domain APIM (F423A) in the ?-Enolase (ENO1) glycolytic enzyme (clone M2)
- Application: Cell culture, immunofluorescence, metabolic function assays
Target Details
- Target: PCNA interacting domain APIM in the ENO1 gene
Applications
- Application: Cell culture, immunofluorescence, metabolic function assays
Handling
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Subculture routine: Cells should be kept at maximum 75% confluency
- Characterisation tests: 6-phospho gluconate dehydrogenase assay, Western Blot, immunofluorescence
Related Tools
- Related tools: ENO 1 F423A M1 HAP1 cell line
