C10 Colorectal Cell Line

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This C10 colorectal cell line was established from surgically resected colorectal adenocarcinomas. Research Area, Cancer, Drug Discovery & Development.

Contributors

Inventor Institute
Walter Bodmer University of Oxford
SKU: 151755
Product description: This C10 colorectal cell line was established from surgically resected colorectal adenocarcinomas. Research Area, Cancer, Drug Discovery & Development.
Conditional: Yes
Production details: This colorectal cell line was established from surgically resected colorectal adenocarcinomas.
Cellosaurus ID: CVCL_5245
Disease: Cancer
Cat. #: 151755
Provenance and Ownership: The C10 Colorectal Cell Line is owned by Cancer Research UK. CancerTools supplies the C10 Colorectal Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing C10 Colorectal Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer; Drug development
Organism: Human
Tissue: Colon
Model: Tumour line
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Iscove’s Modified Dulbecco’s Medium, + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine
Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4×10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. C10 cells grow very slowly; following resuscitation or subculture the cells take at least 48 hours to re-attach. Cells should be left without disturbance during this time to facilitate adhesion. Centrifugation of the cells (100g x 5 min) at resuscitation to remove DMSO improves the establishment of a viable culture. Once attached, the cells grow in discrete islands and use of trypsin or trypsin/EDTA to subculture the cells (even without knocking the flask) yields large clumps. Further disaggregation may be achieved by repeatedly pipetting the cells.
Temperature: 37° C
Atmosphere: 5% CO2
References:

Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.

Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.

Images

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C10 Cell Line - growth profile. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC), UK.
C10 Colorectal Cell Line. 7 days post plating. mage courtesy of the European Collection of Authenticated Cell Cultures (ECACC).

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