#151755

C10 Colorectal Cell Line

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Cat. #151755

C10 Colorectal Cell Line

Cat. #: 151755

Unit size: 1x10^6 cells / vial

Organism: Human

Tissue: Colon

Disease: Cancer

Model: Tumour line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Walter Bodmer

Institute: University of Oxford

Tool Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: C10 Colorectal Cell Line
  • Cancer: Digestive/Gastrointestinal cancer
  • Cancers detailed: Colorectal;Dukes' stage B;Human colorectal adenocarcinoma;Moderately well differentiated
  • Research fields: Cancer;Drug development
  • Organism: Human
  • Tissue: Colon
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Description: The C10 cell line was established from a 71-year old male patient with moderately well differentiated adenocarcinoma of the descending colon classified as Dukes' stage B.
  • Production details: This colorectal cell line was established from surgically resected colorectal adenocarcinomas.
  • Cellosaurus id: CVCL_5245

Handling

  • Format: Frozen
  • Growth medium: Iscove′s Modified Dulbecco′s Medium, + 10% Fetal Bovine Serum (FBS) + 2 mM Glutamine
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37C

References

  • Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.
  • Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.