Cat. #157737
Bovine Type II Alveolar Epithelial (B2AE) cell line
Cat. #: 157737
Unit size: 1x10^6 cells / vial
Availability: 3-4 weeks
Tissue: Alveolus
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Diane Lee
Institute: University of Surrey
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Bovine Type II Alveolar Epithelial (B2AE) cell line
- Research fields: Immunology
- Tissue: Alveolus
- Growth properties: Cobblestone. Fixed (2D), 3D (matrigel) or air-liquid interface (ALI)
- Model: Immortalised Line
- Description: Differentiates into Alveolar Type I (ATI)-like cells when cultured at air-liquid interface, generating a mixed population of Alveolar Type II (ATII) and ATI in a pseudostratified epithelial cell layer. Used in co-culture with bovine pulmonary arterial endothelial cells as a model of the bovine alveolus.
- Production details: Generated by the lentiviral transduction of Bmi1 and hTERT into primary bovine alveolar type II epithelial cells at passage 1. Proliferation rate as for primary.
Handling
- Format: Frozen
- Growth medium: Small airway growth medium (Lonza) or Small airway epithelial cell medium (Promocell). 95 % air/5 % CO2, 37oC humidified incubator. Subculture routine: 0.25 % trypsin/1 mM EDTA 5 minutes at 37oC. Neutralise with medium/10 % FBS. Spin, count and subculture ÄÂÄ? dilution to minimum of 3 x 10,000/cm2. Yield at subculture: ~5 x 10,000/cm2.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: Bovine Alveolar Type II (BATII) cell line
