Cat. #152844
BICR 22 Cell Line
Cat. #: 152844
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Tongue
Disease: Cancer
Model: Tumourigenic cell line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Eric Kenneth Parkinson
Institute: Cancer Research UK Glasgow: The Beatson Institute
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: BICR 22 Cell Line
- Alternate name: bicr22; BICR-22; BICR22; Beatson Institute for Cancer Research 22; HNSCC; SCC-HN
- Cancer: Head and neck cancer
- Cancers detailed: Tongue squamous carcinoma (lymph node metastasis)
- Research fields: Apoptosis and autophagy;Cancer;Drug development;Genetics
- Organism: Human
- Tissue: Tongue
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumourigenic cell line
- Conditional: Yes
- Description: Adherent cell line derived from a lymph node metastasis squamous cell carcinoma of the tongue of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are tumourigenic in athymic mice.
- Additional notes: Amelogenin: X, X CSF1PO: 11 D13S317: 11,13 D16S539: 11,12 D5S818: 11,12 D7S820: 11,12 THO1: 6,7 TPOX: 8 vWA: 16,19
- Cellosaurus id: CVCL_2310
Applications
- Application notes: Amelogenin: X, X CSF1PO: 11 D13S317: 11,13 D16S539: 11,12 D5S818: 11,12 D7S820: 11,12 THO1: 6,7 TPOX: 8 vWA: 16,19
Handling
- Format: Frozen
- Growth medium: DMEM + 2mM Glutamine + 2% Foetal Bovine Serum (FBS) + 0.4 µg/ml Hydrocortisone.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Subculture routine: Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.05% trypsin/EDTA; 8% CO₂; 37°C. Suggested seeding density 2 x 10,000 cells/cm². Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells. Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37°C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 10^4 cells/cm².
Related Tools
- Related tools: BICR 10 Cell Line ; BICR 16 Cell Line ; BICR 18 Cell Line ; BICR 3 Cell Line ; BICR 56 Cell Line ; BICR 6 Cell Line ; BICR 78 Cell Line ; BICR 31 Cell Line ; BICR 82 Cell Line
References
- Loughran et al. 1997. Oncogene. 14(16):1955-1964. PMID: 9150362.
- Edington et al. 1995. Mol Carcinog. 13(4):254-265. PMID: 7646764.
