#161850

1BR PBRM1 KO5

Cat. #161850

1BR PBRM1 KO5

Cat. #: 161850

Availability: 8-10 weeks

Organism: Human

Tissue: skin

Model: Genetically modified ; immortalised non-cancer cells

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jessica Downs, Hugang Feng

Institute: The Institute of Cancer Research

Primary Citation: Feng et. al. 2022 Genes Dev 36:790-806 PMID: 35902118

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

  • Name: 1BR PBRM1 KO5
  • Parental cell: 1BR3-hTERT
  • Clone: 5
  • Organism: Human
  • Gender: Male
  • Tissue: skin
  • Morphology: Fibroblast
  • Growth properties: Adherent
  • Model: Genetically modified ; immortalised non-cancer cells
  • Model description: PBRM1 knockout
  • Crispr: Yes
  • Conditional: No
  • Products or characteristics of interest: Gene loss-of-function, chromatin-remodelling, DNA damage, cell cycle response, regulation of t-cell-mediated cell killing.
  • Description: 1BR3-hTERT cell line knockout for PBRM1 (clone 5). This cell line was generated using CRISPR-Cas9 engineering to create a loss of function mutation in the indicated subunit of the SWI/SNF chromatin remodelling complex. The cell line was validated using Sanger sequencing, immunofluorescence, and proteomic analysis.
  • Application: Chromatin remodelling biology studies
  • Biosafety level: 1

Applications

  • Application: Chromatin remodelling biology studies

Handling

  • Format: Frozen
  • Growth medium: DMEM + 10S + 1% Penicillin/Streptomycin
  • Temperature: 37C
  • Atmosphere: 5% CO2 in air
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Cultured in antibiotics: 1% Penicillin/Streptomycin
  • Str profiling: See Cellosaurus profile of parental cell line

References

  • Feng et. al. 2022 Genes Dev 36:790-806 PMID: 35902118