Cat. #153752
16E6-E7 HFK
Cat. #: 153752
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Disease: Cancer
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Aloysius Klingelhutz
Institute: The University of Iowa
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: 16E6-E7 HFK
- Research fields: Cancer
- Tool sub type: Continuous
- Parental cell: Human foreskin keratinocytes (HFKs) established from foreskin tissue obtained from newborns
- Organism: Human
- Disease: Cancer
- Model: Immortalised Line
- Conditional: No
- Description: Expresses skin cell markers; proinflammatory. Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
- Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
- Production details: HPV-16 E7 expressing keratinocytes were generated by infecting normal primary cells with an LXSH retrovirus that expressed E7 and conferred hygromycin resistance. After selection in hygromycin, cells were infected with an LXSN retroviral construct that expressed wild-type HPV-16 E6 and conferred G418 resistance.ÄÂ?Â
- Biosafety level: 1
Target Details
- Target: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
Applications
- Application: Used as an immortal neonatal human skin keratinocyte line for pathogenesis and inflammation studies
Handling
- Format: Frozen
- Growth medium: Maintained in Keratinocyte-SFM (Gibco-BRL, Rockville, MD). G418 selection.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
References
- Duffy et al. 2003. Virology. 314(1):196-205. PMID: 14517073.
