The TagRFP polycloncal antibody is one of the Brainbow collection. The antibody host species is a Chicken. TagRFP is a red fluorescent protein generated from Entacmaea quadricolor._x000D_ _x000D_ The brainbow collection has been used in a multicolour labelling strategy for the fluorescent imaging of neuronal circuits and individual neurons in mice, drosophila and zebrafish and […]
| Inventor | Institute |
|---|---|
| Dawen Cai | University of Michigan, USA |
| Cat. #: | 155265 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 μl |
| Research Fields: | Neurobiology;Neurobiology |
| Application: | IF ; IP ; WB |
| Target: | TagRFP |
| Host: | Chicken |
| Class: | Polyclonal |
| Primary citation: | Cai et al. 2013. Nat Methods. 10(6):540-547. PMID: 23817127. |
| Alternate name: | Anti-TagRFP (Chicken) |
|---|---|
| Product description: | The TagRFP polycloncal antibody is one of the Brainbow collection. The antibody host species is a Chicken. TagRFP is a red fluorescent protein generated from Entacmaea quadricolor. The brainbow collection has been used in a multicolour labelling strategy for the fluorescent imaging of neuronal circuits and individual neurons in mice, drosophila and zebrafish and non-neuronal cells in mice. The Brainbow toolkit allows scientists to image highly complex tissue structures by relying on a stochastic method for making different expression ratio combinations of fluorescent proteins so that structures in proximity can be resolved. |
| Additional notes: | To minimise background, the sample section thickness should be less than the working distance of the objective, generally <100 μm for high numerical aperture lenses. |
| Target background: | The TagRFP is a fluorescent proteins originated from sea anemone (Entacmaea quadricolor) |
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| Format: | Liquid |
|---|---|
| Storage buffer: | Serum with 0.02% sodium azide |
| Storage conditions: | Best stored at -80°C for long-term storage (>3 years) |
| Shipping conditions: | Dry ice |
| References: |
Roossien et al. 2019. Bioinformatics. 35(18):3544-3546. PMID: 30715234. Chen et al. 2018. Mol Psychiatry. 23(7):1614-1625. PMID: 28761082. Takesian et al. 2018. Nat Neurosci. 21(2):218-227. PMID: 29358666. Roossien et al. 2017. Methods Mol Biol. 1642:211-228. PMID: 28815503. Manent et al. 2017. Oncogene. 36(40):5576-5592. PMID: 28581519. Chang et al. 2017. Nat Methods. 14(6):593-599. PMID: 28417997. Zanca et al. 2017. Genes Dev. 31(12):1212-1227. PMID: 28724615. Tillberg et al. 2016. Nat Biotechnol. 34(9):987-992. PMID: 27376584. Cai et al. 2013. Nat Methods. 10(6):540-547. PMID: 23817127. |
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