Monoclonal antibody for investigation into hereditary spastic paraplegias.
| Inventor | Institute |
|---|---|
| Giampietro Schiavo | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 151424 |
|---|---|
| Tool sub type: | Primary antibody |
| Unit size: | 100 ug |
| Research Fields: | Cell biology;Cell signaling and signal transduction;Genetics;Metabolism;Neurobiology;Tissue-specific biology |
| Application: | ELISA ; IF ; WB |
| Target: | Spastin |
| Reactivity: | Human ; Mouse ; Rat |
| Clone: | Sp 6C6 |
| Host: | Mouse |
| Class: | Monoclonal |
| Product description: | Monoclonal antibody for investigation into hereditary spastic paraplegias. |
|---|---|
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Molecular weight: | 52 kDa |
| Immunogen: | The Anti-Spastin antibody [Sp 6C6] was raised against a recombinant His6- tagged human spastin representing a common splice variant of the gene, starting from the second ATG and missing exon 4. The resulting recombinant protein is 509 amino acids long and has an apparent molecular weight of 55 KDa. |
| Immunogen Uniprot ID: | Q9UBP0 |
| Myeloma used: | Sp2/0-Ag14 |
| Target background: | Spastin is thought have a role in microtubule dynamics through its function as a microtubule-severing protein. It is localised to the centrosome of neuronal cells but is not found in glial cells. Spastin is involved in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutation in the ATPase binding domain of spastin causes hereditary spastic paraplegias (HSP), a large group of clinically similar disorders. Mutations within spastin cause the most common form of autosomal dominant spastic paraplegia 4. Mutant forms of spastin are generally found throughout the cytoplasm rather than within the nucleus. There are two splice isoforms of spastin (one without exon4) and two alternative ATG start sites, which may determine the localisation of the translate protein, coded by the SPAST gene. |
|---|
| Format: | Liquid |
|---|---|
| Concentration: | 1 mg/ml |
| Storage buffer: | PBS with 0.02% azide |
| Storage conditions: | Store at -20° C frozen. Avoid repeated freeze / thaw cycles |
| Shipping conditions: | Dry ice |
| References: |
Salinas et al. 2005. J Neurochem. 95(5):1411-20. PMID: 16219033. Human spastin has multiple microtubule-related functions. |
|---|
| Cat. # | Tool Name | |||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 151030 | Anti-Progesterone [11P14] |
Key Info
Anti-Progesterone [11P14]
|
View Tool | |||||||||||||||||||
| 151022 | Anti-IL12 [1-1A4] |
Key Info
Anti-IL12 [1-1A4]
|
View Tool | |||||||||||||||||||
| 151033 | Anti-HSVUL42 [13C9] |
Key Info
Anti-HSVUL42 [13C9]
|
View Tool | |||||||||||||||||||
| 151040 | Anti-CD11 & CD18 [24] |
Key Info
Anti-CD11 & CD18 [24]
|
View Tool | |||||||||||||||||||
| 151035 | Anti-CD8 [14] mAb |
Key Info
Anti-CD8 [14] mAb
|
View Tool | |||||||||||||||||||
Please note we may take up to three days to respond to your enquiry.
CancerTools.org uses the contact information provided to respond to you about our research tools and service. For more information please review our privacy policy.