Cat. #151107
Anti-PLAP [HD 8B6]
Cat. #: 151107
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: Placental Alkaline Phosphatase (PLAP), Regan and Nagao isoenzymes.
Class: Monoclonal
Application: IF ; ELISA ; IHC ; RIA
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Walter Bodmer
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-PLAP [HD 8B6]
- Research fields: Cancer;Cell biology
- Clone: HD 8B6
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Strain: Balb/c
- Reactivity: Human
- Host: Mouse
- Application: IF ; ELISA ; IHC ; RIA
- Description: HD 8B6 has potential uses in the diagnosis of ovarian and testicular tumours. The antibody detects both Regan and Nagao isoenzymes.
- Immunogen: Fresh Hep-2 cells followed by booster injections of fresh Hep-2 cells that have had their surface expression of PLAP boosted in culture.
- Immunogen uniprot id: P05187
- Isotype: IgG2a
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: Placental Alkaline Phosphatase (PLAP), Regan and Nagao isoenzymes.
- Target background: Alkaline phosphatases are phosphodiesterases. The placental-specific isozyme of Alkaline Phosphatase (PLAP) is found in trophoblast cells of normal human mature placenta, seminomas of testis and ovarian carcinomas. The detection of alkaline phosphatase in serum is a marker for ovarian and testicular cancer.
Applications
- Application: IF ; ELISA ; IHC ; RIA
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Dry ice
References
- Sharp et al. 2010. PLoS One. 5(10):e13130. PMID: 20976190.
- Inhibition of cellular protein secretion by norwalk virus nonstructural protein p22 requires a mimic of an endoplasmic reticulum export signal.
- Wojtowicz et al. 2007. Cell. 130(6):1134-45. PMID: 17889655.
- A vast repertoire of Dscam binding specificities arises from modular interactions of variable Ig domains.
- Durbin et al. 1988. Int J Cancer Suppl. 2:50-8. PMID: 2450848.
- Durbin et al. 1988. Int J Cancer Suppl. 2:59-66. PMID: 3162446.
- Production of monoclonal antibodies to placental alkaline phosphatase: preliminary characterisation includes identification of one antibody reactive with routinely fixed histological preparations.
- Monoclonal antibodies to placental alkaline phosphatase: preclinical evaluation in a human xenograft tumour model of F(ab')2 and Fab fragments.
