Cat. #151587
Anti-Phospho RacGAP1 (Ser157) [pS157 HsCyk-4]
Cat. #: 151587
Sub-type: Primary antibody
Unit size: 100 ug
Target: pS157 HsCyk-4 (MgcRacGAP and RacGAP1)
Class: Polyclonal
Application: IF ; IP ; WB
Reactivity: Human ; Mouse ; Rat
Host: Rabbit
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Mark Petronczki
Institute: Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Phospho RacGAP1 (Ser157) [pS157 HsCyk-4]
- Alternate name: Cyk-4
- Research fields: Cell biology
- Clone: pS157 HsCyk-4
- Tool sub type: Primary antibody
- Class: Polyclonal
- Conjugation: Unconjugated
- Reactivity: Human ; Mouse ; Rat
- Host: Rabbit
- Application: IF ; IP ; WB
- Description: HsCyk-4 (or MgcRacGAP) is a subunit of the centralspindlin, and contains a GTPase activator protein (GAP) domain and directly interacts with the GTP exchange factor (GEF) Ect2, and both HsCyk-4 and Ect2 are essential for equatorial recruitment of the RhoA GTPase, the master regulator of actomyosin dynamics during cytokinesis. Plk1 binds and directly phosphorylates the HsCyk-4.
- Immunogen: Synthetic phospho-peptide ILSDI-pS-FDKTD corresponding to HsCyk-4 aa 152-162 (human)
- Recommended controls: Western blot on mitotic HeLa cell lysates (e.g. nocodazole arrested cells)
Target Details
- Target: pS157 HsCyk-4 (MgcRacGAP and RacGAP1)
- Tissue cell line specificity: Western blot on mitotic HeLa cell lysates (e.g. nocodazole arrested cells)
- Target background: HsCyk-4 (or MgcRacGAP) is a subunit of the centralspindlin, and contains a GTPase activator protein (GAP) domain and directly interacts with the GTP exchange factor (GEF) Ect2, and both HsCyk-4 and Ect2 are essential for equatorial recruitment of the RhoA GTPase, the master regulator of actomyosin dynamics during cytokinesis. Plk1 binds and directly phosphorylates the HsCyk-4.
Applications
- Application: IF ; IP ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Unit size: 100 ug
- Storage conditions: -80° C
- Shipping conditions: Shipping at 4° C
