Anti-integrin a3 inhibiting

Tool Details

*FOR RESEARCH USE ONLY

• Name: Anti-integrin a3 inhibiting
• Research fields: Cell biology
• Clone: P1B5
• Tool sub type: Primary antibody
• Class: Monoclonal
• Molecular weight: 119kDa
• Reactivity: Human
• Host: Mouse
• Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Description: Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis. Integrin ?3 (P1B5) is a mouse monoclonal antibody developed to identify four distinct classes (I, II, III, IV) of cell surface receptors for native collagen. P1B5 inhibited attachment to type VI and I collagens and partially inhibited attachment to fibronectin and laminin as well.
• Immunogen: HT1080 human fibrosarcoma cell line
• Isotype: IgG1

Target Details

• Target: Integrin alpha-3 (CD49b)
• Molecular weight: 119kDa
• Target background: ?2?1 complex of Collagen receptors: I, II, III, IV. P1B5 inhibits cell adhesion to collagen and fibronectin.

Applications

• Application: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

• Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.

References

• Wayner et al.1987. Journal of Cell Biology.105(4):1873-84.PMID: 2822727
• Carter et al.1990. J Cell Biol.111(6 ):3141-54.PMID: 2269668
• Symington et al. 1993.J Cell Biol.120(2):523-35.PMID: 8421064