Cat. #153598
Anti-F-actin [NH3]
Cat. #: 153598
Unit size: 100 ug
Availability: 3-4 weeks
Target: Filamentous Actin
Class: Monoclonal
Application: ELISA ; FACS
Reactivity: Chicken ; Human ; Mouse ; Rabbit
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Lynda Partridge
Institute: University of Sheffield
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-F-actin [NH3]
- Alternate name: Actin Antibody, Anti-Actin Antibody, F-Actin, f actin antibody, Filamentous actin antibody
- Research fields: Cell biology;Developmental biology
- Clone: NH3
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Chicken ; Human ; Mouse ; Rabbit
- Host: Mouse
- Application: ELISA ; FACS
- Description: Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types. Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells.
- Immunogen: Human monocytes and the human monocyte-like cell line, U937.
- Immunogen uniprot id: P60709
- Isotype: IgM kappa
Target Details
- Target: Filamentous Actin
- Target background: Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells.
Applications
- Application: ELISA ; FACS
Handling
- Format: Liquid
- Concentration: 1mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163
