Cat. #151464
Anti-Cisplatin modified DNA [CP9/19]
Cat. #: 151464
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 1-2 weeks
Target: Cisplatin modified native DNA
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IP ; DB
Host: Rat
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Michael Tilby
Institute: The Institute of Cancer Research; Newcastle University
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-Cisplatin modified DNA [CP9/19]
- Cancers detailed: Broadly Applicable;Chemotherapy
- Research fields: Cancer;Genetics
- Clone: CP9/19
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Host: Rat
- Application: ELISA ; FACS ; IHC ; IP ; DB
- Description: Antibody CP9/19 recognises only the intra-strand cross-links formed by cisplatin between adjacent purines.
- Immunogen: Cisplatin modified native DNA
- Isotype: IgG2a
- Myeloma used: Y3.AG.1.2.3
- Recommended controls: RNA/DNA
Target Details
- Target: Cisplatin modified native DNA
- Tissue cell line specificity: RNA/DNA
- Target background: This antibody enables the quantification of cisplatin-induced adducts on DNA. This antibody has also been recently used for isolation of DNA fragments carrying adducts to enhance the sensitivity of subsequent PCR-based analyses and is central to ongoing studies of variation in the nature of cisplatin adducts formed in different cell lines.
Applications
- Application: ELISA ; FACS ; IHC ; IP ; DB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
Related Tools
- Related tools: Anti-Cisplatin modified DNA, Recombinant [CP9/19]
References
- Powell et al. 2015. Sci Rep. 5:7975. PMID: 25609656.
- 3D-DIP-Chip: a microarray-based method to measure genomic DNA damage.
- Kothandapani et al. 2012. Exp Cell Res. 318(16):1973-86. PMID: 22721696.
- Downregulation of SWI/SNF chromatin remodeling factor subunits modulates cisplatin cytotoxicity.
- Kothandapani et al. 2011. J Biol Chem. 286(16):14564-74. PMID: 21357694.
- Novel role of base excision repair in mediating cisplatin cytotoxicity.
- Meczes et al. 2005. Biochem Pharmacol. 70(12):1...
