Anti-Cdk1 [17 (A17)]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-Cdk1 [17 (A17)]
• Alternate name: Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; Cell Division Control Protein 2 Homolog; Cell Division Protein Kinase 1; P34 Protein Kinase; P34CDC2; CDC28A; CDC2; Cell Cycle Controller CDC2; CDKN1
• Research fields: Cancer;Cell biology;Neurobiology
• Clone: 17 (A17)
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 34 kDa
• Strain: Balb/c
• Reactivity: Chicken ; Human ; Mouse ; Rat ; Xenopus laevis
• Host: Mouse
• Application: ELISA ; IHC ; IP ; WB
• Description: Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.
• Immunogen: A carboxy-terminal fragment starting at methionine 85 of p34 cdc2 (Xenopus laevis).
• Isotype: IgG2a
• Myeloma used: Sp2/0-Ag14
• Recommended controls: Tonsil

Target Details

• Target: Cyclin dependent kinase 1 (Cdk1, cdc2)
• Molecular weight: 34 kDa
• Tissue cell line specificity: Tonsil
• Target background: Cdk1 (Cdc2) is a serine/threonine kinase which forms complexes with cyclins A and B which then phosphorylate a variety of target substrates to enable cell cycle progression. Therefore, Cdk1 is a marker for proliferating tumour cells.

Applications

• Application: ELISA ; IHC ; IP ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Zhou et al. 2015. Platelets. :1-11. PMID: 26325015.
• Platelets promote cartilage repair and chondrocyte proliferation via ADP in a rodent model of osteoarthritis.
• Coulonval et al. 2011. Mol Biol Cell. 22(21):3971-85. PMID: 21900495.
• Coupling of T161 and T14 phosphorylations protects cyclin B-CDK1 from premature activation.
• Gannon et al. 1998. Genes Cells. 3(1):17-27. PMID: 9581979.
• A measure of the mitotic index: studies of the abundance and half-life of p34cdc2 in cultured cells and normal and neoplastic tissues.
• Goodger et al. 1996. J Pathol. 178(4):422-8. PMID: 8691321.
• The localization of p34cdc2 in the cells of normal, hyperplastic, and malignant epithelial and lymphoid tissues of the oral cavity.
• Doussis-Anagnostopoulou et al. 1994. Histopathology. 24(4):335-40. PMID: 8045523.
• Distribution of the cdc2 gene product in normal tissues: an immunocytochemical study using four new monoclonal antibodies.
• Kobayashi et al. 1992. Mol Biol Cell. 3(11):1279-94. PMID: 1333843.
• Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits.