Cat. #151376
Anti-CD79b [B29/123] mAb
Cat. #: 151376
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-4 weeks
Target: CD79b
Class: Monoclonal
Application: IHC ; WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Jacqueline Cordell
Institute: University of Oxford
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD79b [B29/123] mAb
- Alternate name: CD79b Molecule; CD79B Antigen (Immunoglobulin-Associated Beta); Immunoglobulin-Associated B29 Protein; B-Cell-Specific Glycoprotein B29; Ig-Beta
- Research fields: Cell signaling and signal transduction;Immunology
- Clone: B29/123
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: IHC ; WB
- Description: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.
- Immunogen: Synthetic peptide representing the c-terminus from residue 215 of the murine B29 polypeptide.
- Isotype: IgG2b
- Myeloma used: P3/NS1/1-Ag4.1
Target Details
- Target: CD79b
- Target background: The B-cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a (mb1) and CD79b / B29 polypeptides which are non-covalently associated with membrane bound immunoglobulins on B-cells. CD79a first appears at pre B-cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B-cell lymphomas, in B-cell lines, the majority of acute leukemias of precursor B-cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). The induction of tyrosine kinase activity after antigen binding leads to phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signalling.
Applications
- Application: IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Hauser et al. 2013. Mol Immunol. 54(3-4):247-53. PMID: 23318223.
- Broad feedback inhibition of pre-B-cell receptor signaling components.
- Ouchida et al. 2010. J Immunol. 185(1):294-301. PMID: 20519653.
- A role for lysosomal-associated protein transmembrane 5 in the negative regulation of surface B cell receptor levels and B cell activation.
- Lankester et al. 1994. J Immunol. 152(5):2157-62. PMID: 8133032.
- Evidence for a direct physical interaction of membrane IgM, IgD, and IgG with the B29 gene product.
- Jones et al. 1993. J Immunol. 150(12):5429-35. PMID: 8515069.
- Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies.
- Brouns et al. 1993. Eur J Immunol. 23(5):1088-97. PMID: 8477803.
- The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction.
- Mason et al. 1992. Eur J Immunol. 22(10):2753-6. PMID: 1396979.
- The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation.