Anti-CD79a [HM47] mAb

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD79a [HM47] mAb
• Alternate name: B-cell antigen receptor complex-associated protein alpha chain; Ig-alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein
• Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Stem cell biology
• Clone: HM47
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Reactivity: Bovine ; Chicken ; Horse ; Human ; Guinea Pig ; Mouse ; Opossum ; Pig ; Primate ; Rat ; Rabbit
• Host: Mouse
• Application: IHC ; IP ; WB
• Description: The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections.
• Immunogen: Synthetic peptide of 14 amino acids representing amino acids 202-216 of the human mb-1 cDNA sequence
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1

Target Details

• Target: CD79a
• Target background: The B cell Antigen receptor constitutes a disulphide linked heterodimer, consisting of CD79a / mb1 and CD79b / B29 polypeptides, non covalently associated with membrane bound immunoglobulins on B cells. CD79a first appears at pre B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in B cell lymphomas, in B cell lines, the majority of acute leukemias of precursor B cell type and in some myelomas. The CD79a/b heterodimer interacts with at least one tyrosine kinase (Lyn). Induction of tyrosine kinase activity after antigen binding causes phosphorylation of the CD79a/b dimer, and also of other molecules, thereby initiating intracellular signaling. CD79a is widely used as an adjunct to CD20 for detecting normal and neoplastic B cells in tissues sections.

Applications

• Application: IHC ; IP ; WB

Handling

• Format: Liquid
• Concentration: 0.9-1.1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: -15° C to -25° C
• Shipping conditions: Dry ice

References

• Lankester et al. 1994. J Immunol. 152(5):2157-62. PMID: 8133032.
• Evidence for a direct physical interaction of membrane IgM, IgD, and IgG with the B29 gene product.
• Brouns et al. 1993. Eur J Immunol. 23(5):1088-97. PMID: 8477803.
• The structure of the mu/pseudo light chain complex on human pre-B cells is consistent with a function in signal transduction.
• Mason et al. 1991. J Immunol. 147(11):2474-82. PMID: 1747162.
• The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells.
• van Noesel et al. 1991. J Immunol. 146(11):3881-8. PMID: 2033258.
• The membrane IgM-associated heterodimer on human B cells is a newly defined B cell antigen that contains the protein product of the mb-1 gene.