Cat. #152483
Anti-CD49b [16B4]
Cat. #: 152483
Sub-type: Primary antibody
Unit size: 100 ug
Availability: 3-5 days
Target: CD49f (Integrin a2+b1)
Class: Monoclonal
Application: ELISA ; FACS ; IHC ; IF ; IP
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Martin Humphries
Institute: University of Manchester
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Anti-CD49b [16B4]
- Alternate name: Integrin Subunit Alpha 2; Alpha 2 Subunit Of VLA-2 Receptor; Platelet Membrane Glycoprotein Ia; CD49 Antigen-Like Family Member B; Collagen Receptor; CD49B; GPIa; Very Late Activation Protein 2 Receptor; Alpha-2 Subunit; Human Platelet Alloantigen System; Platelet Glycoprotein GPIa; VLA-2 Subunit Alpha; CD49b Antigen; HPA-5; VLA-2; VLAA2; BR
- Research fields: Cell biology;Cell signaling and signal transduction;Immunology
- Clone: 16B4
- Tool sub type: Primary antibody
- Class: Monoclonal
- Conjugation: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; FACS ; IHC ; IF ; IP
- Description: CD49b is an integrin alpha subunit which makes up half of the ÄÂ?2ÄÂ?1 integrin duplex. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells (the NKT cells), NK cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling.
- Immunogen: Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
- Isotype: IgG1
- Myeloma used: P3X63Ag8.653
- Recommended controls: In IF, this antibody gave a positive signal in HepG2 cells.
Target Details
- Target: CD49f (Integrin a2+b1)
- Tissue cell line specificity: In IF, this antibody gave a positive signal in HepG2 cells.
- Target background: CD49b is an integrin alpha subunit which makes up half of the a2?1 integrin duplex. Integrins are heterodimeric integral membrane glycoproteins composed of a distinct alpha chain and a common beta chain. They are found on a wide variety of cell types including, T cells (the NKT cells), NK cells, fibroblasts and platelets. Integrins are involved in cell adhesion and also participate in cell-surface mediated signalling.
Applications
- Application: ELISA ; FACS ; IHC ; IF ; IP
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Unit size: 100 ug
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15° C to -25° C
- Shipping conditions: Shipping at 4° C
References
- Suarez et al. 2015. Arch Dermatol Res. 307(2):115-33. PMID: 25322916.
- Menhofer et al. 2014. Cardiovasc Res. 104(2):303-14. PMID: 25239826.
- Identification of biomarkers involved in differential profiling of hypertrophic and keloid scars versus normal skin.
- In vitro and in vivo characterization of the actin polymerizing compound chondramide as an angiogenic inhibitor.
- Tuckwell et al. 2000. Biochem J. 350 Pt 2:485-93. PMID: 10947963.
- Monoclonal antibodies identify residues 199-216 of the integrin alpha2 vWFA domain as a Fnly important region within alpha2beta1.
- Sincock et al. 1999. J Cell Sci. 112 ( Pt 6):833-44. PMID: 10036233.
- PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
- Fitter et al. 1999. Biochem J. 338 ( Pt 1):61-70. PMID: 9931299.
- Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
