Anti-CD3 monoclonal antibody [UCH-T1TM]

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Anti-CD3 monoclonal antibody [UCH-T1TM]
• Alternate name: T3 complex
• Research fields: Cancer;Cell biology;Cell signaling and signal transduction;Immunology;Stem cell biology
• Clone: UCH-T1
• Tool sub type: Primary antibody
• Class: Monoclonal
• Conjugation: Unconjugated
• Molecular weight: 52 kDa
• Strain: Balb/c
• Reactivity: Human
• Host: Mouse
• Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB
• Description: CRT trademarked famous anti-CD3 monoclonal antibody, capable of differentiating between T vs B cells lymphomas and leukaemia's.
• Immunogen: Human infant thymocytes and Sezary cells.
• Immunogen uniprot id: P04234
• Isotype: IgG1
• Myeloma used: P3/NS1/1-Ag4.1
• Additional notes: UCH-T1TM is a registered trademark of Cancer Research Technology, Limited. All rights reserved.

Target Details

• Target: CD3
• Molecular weight: 52 kDa
• Target background: The CD3 complex, composed of four distinct CD3 polypeptide chains (CD3?, CD3d and 2X CD3), associates with the T cell antigen receptor (TCR). It is found on all mature human T lymphocytes, NK cells and some thymocytes. CD3 is a member of the immunoglobulin superfamily, involved in antigen recognition, T lymphocyte activation and signal transduction. UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia's. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Szary leukaemia's and approximately 70% of acute lymphoblastic leukaemia's of T cell origin. It can also be used to study the role of CD3 in TCR signal transduction events. This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunised against human lymphocytes, with UCHT1 being one of the first successful fusions.

Applications

• Application: FACS ; IHC ; IF ; IP ; Fn ; RIA ; WB

Handling

• Format: Liquid
• Concentration: 1 mg/ml
• Unit size: 100 ug
• Storage buffer: PBS with 0.02% azide
• Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
• Shipping conditions: Dry ice

Related Tools

• Related tools: Anti-CD3, Recombinant [UCH-T1TM]

References

• Bencsikova et al. 2019. BMC Cancer. 19(1):687. PMID: 31307428.
• Oll Hurtado et al. 2019. PLoS One. 14(8):e0216373. PMID: 31398192.
• Acquaviva et al. 2019. Front Immunol. 10:1922. PMID: 31474991.
• rsek et al. 2012. J Immunol. 189(4):1602-10. PMID: 22798681.
• CD3-chain expression of human T lymphocytes is regulated by TNF via Src-like adaptor protein-dependent proteasomal degradation.
• Muhammad et al. 2009. J Immunol. 182(12):7672-80. PMID: 19494291.
• Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome.
• Nishi et al. 2008. Glycobiology. 18(12):1065-73. PMID: 18796645.
• Fn and structural bases of a cysteine-less mutant as a long-lasting substitute for galectin-1.
• Dornan et al. 2002. J Biol Chem. 277(3):1912-8. PMID: 11694532.
• Differential association of CD45 isoforms with CD4 and CD8 regulates the actions of specific pools of p56lck tyrosine kinase in T cell antigen receptor signal transduction.
• Lafont et al. 1999. J Biol Chem. 274(36):25743-8. PMID: 10464312.
• Evidence for a p21(ras)/Raf-1/MEK-1/ERK-2-independent pathway in stimulation of IL-2 gene transcription in human primary T lymphocytes.
• Salmern et al. 1991. J Immunol. 147(9):3047-52. PMID: 1717585.
• A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.
• Kanellopoulos et al. 1983. EMBO J. 2(10):1807-14. PMID: 6227478.
• Biosynthesis and molecular nature of the T3 antigen of human T lymphocytes.
• Garson et al. 1982. Nature. 298(5872):375-7. PMID: 6178042.
• Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.
• Beverley et al. 1981. Eur J Immunol. 11(4):329-34. PMID: 6788570.
• Distinctive Fn characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody.

Documentation

• Product datasheet