Cat. #153627
Anti-mtEF-Tu
Cat. #: 153627
Unit size: 100 ug
Availability: 10-12 weeks
Target: Mitochondrial EF-Tu
Class: Monoclonal
Application: WB
Reactivity: Human
Host: Mouse
£300.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Zofia Chrzanowska-Lightowlers
Institute: Newcastle University
Tool Details
*FOR RESEARCH USE ONLY
- Name: Anti-mtEF-Tu
- Alternate name: Mitochondrial Elongation Factor Tu, EF-Tu, P43, Elongation Factor Tu, Mitochondrial, EF-TuMT, COXPD4, EFTU
- Class: Monoclonal
- Conjugation: Unconjugated
- Molecular weight: Predicted 43 kDa
- Reactivity: Human
- Host: Mouse
- Application: WB
- Description: Two elongation factors, EF-Tu and EF-Ts, participate in the elongation phase during protein biosynthesis on the human mitoribosome. The mitochondrial EF-Tu promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Mutations identified in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy
- Immunogen: Recombinant protein
Target Details
- Target: Mitochondrial EF-Tu
- Molecular weight: Predicted 43 kDa
- Target background: Two elongation factors, EF-Tu and EF-Ts, participate in the elongation phase during protein biosynthesis on the human mitoribosome. The mitochondrial EF-Tu promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Mutations identified in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy
Applications
- Application: WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1mg/ml
- Unit size: 100 ug
- Storage buffer: Thaw in RPMI + 10% condimed + 20% FCS + 1x pen and Strep + 1 x L-glutamine (~2.7mM). Once growing serially dilute and reduce condimed from 10% to 5% to 2% to 0%. After that can start to drop FCS from 20% to 15% to 10% or even as low as 5%. When growing sufficiently, then stop feeding, when approx. 80% dead cells look shriveled and medium goes yellowish, then pellet the cells down hard and retain the supernatant. This is then filtered to remove as much non-antibody as possible and used as anti-serum.
- Storage conditions: 4° C
- Shipping conditions: Shipping at 4° C