#153794

pTB_CFTR_Ex9 155T vector

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Cat. #153794

pTB_CFTR_Ex9 155T vector

Cat. #: 153794

Sub-type: pBluescript KS

Availability: Please enquire for quantities and pricing

Target: CFTR Exon 9 minigene

Bacterial Resistance: Ampicillin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Prof Emanuele Buratti

Institute: International Centre For Genetic Engineering And Biotechnology (ICGEB)

Tool Details
Target Details
Application Details
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pTB_CFTR_Ex9 155T vector
  • Alternate name: Cystic Fibrosis Transmembrane Conductance Regulator, Channel Conductance-Controlling ATPase
  • Research fields: Genetics
  • Tool sub type: pBluescript KS
  • Backbone size without insert: 2958
  • Bacterial resistance: Ampicillin
  • Description: Concentration 2mg/ml
  • Additional notes: This minigene construct consists of a minimal α-globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation.

Target Details

  • Target: CFTR Exon 9 minigene

Application Details

  • Application notes: Concentration 2mg/ml

References

  • D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.
  • Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.
  • Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.
  • Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.