Cat. #153794
pTB_CFTR_Ex9 155T vector
Cat. #: 153794
Sub-type: pBluescript KS
Availability: Please enquire for quantities and pricing
Target: CFTR Exon 9 minigene
Bacterial Resistance: Ampicillin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Prof Emanuele Buratti
Institute: International Centre For Genetic Engineering And Biotechnology (ICGEB)
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: pTB_CFTR_Ex9 155T vector
- Alternate name: Cystic Fibrosis Transmembrane Conductance Regulator, Channel Conductance-Controlling ATPase
- Research fields: Genetics
- Tool sub type: pBluescript KS
- Backbone size without insert: 2958
- Bacterial resistance: Ampicillin
- Description: This minigene construct consists of a minimal alpha globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation. Concentration 2ug/ml
- Additional notes: This minigene construct consists of a minimal alpha globin promoter and SV40 enhancer which drive the transcription of the minigene. Downstream, an alpha-globin-fibronectin EDB minigene is present with a unique NdeI site where a fragment that contains exon 9 (183bp) along with part of the flanking introns was inserted. The exon 9 sequence also carries a C155T mutation that destroys a splicing enhancer within its sequence. This means that when transfected into cells the exon 9 is included approximately in 50% of the transcripts. In this manner, it is possible to see changes both with regards to upregulation of exon inclusion or its downregulation.
Target Details
- Target: CFTR Exon 9 minigene
Application Details
- Application notes: Concentration 2ug/ml
References
- D'Ambrogio et al. 2009. Nucleic Acids Res. 37(12):4116-26. PMID: 19429692.
- Functional mapping of the interaction between TDP-43 and hnRNP A2 in vivo.
- Pagani et al. 2003. J Biol Chem. 278(29):26580-8. PMID: 12732620.
- Missense, nonsense, and neutral mutations define juxtaposed regulatory elements of splicing in cystic fibrosis transmembrane regulator exon 9.
