#160576

pROSA26-GNrep vector

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Cat. #160576

pROSA26-GNrep vector

Cat. #: 160576

Sub-type: pROSA26

Availability: Please enquire for quantities and pricing

Bacterial Resistance: Neomycin

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Dr Claudio Franco

Institute: Instituto de Medicina Molecular JoĂŁo Lobo Antunes

Tool Details
Application Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: pROSA26-GNrep vector
  • Research fields: Developmental biology
  • Tool sub type: pROSA26
  • Backbone size without insert: 1424bp
  • Bacterial resistance: Neomycin
  • Description: This vector was used to generate a reporter mouse for front-rear cell polarity (mouse linked above in the "Related" tab).
  • Additional notes: As a backbone for this vector, pROSA26â€�DV2 vector that carries Rosa26 5′â€� and 3′â€�homology arms and enables rapid, modular, and efficient generation of plasmid DNA suitable for homologous recombination into the Rosa26 genomic locus of mouse embryonic stem cells (mESCs) was used (Nyabi et al., 2009, PMID:19279185). This plasmid allows for multisite Gateway recombinationâ€�mediated insertion of three entry DNA sequences which was used here to generate pROSA26â€�GNrep vector, containing a CAGGS synthetic promoter followed by a loxPâ€�dSTOPâ€�loxP cassette, the GNrep cassette, by a selection cassette pAâ€�FRTâ€�PGKâ€�Neoâ€�pAâ€�FRT (linked in "Related" above).

Application Details

  • Application notes: This vector was used to generate a reporter mouse for front-rear cell polarity (mouse linked above in the "Related" tab).

Related Tools

  • Related tools: GNrep mouse

References

  • Barbacena et al. 2019. Genesis. 57(6):e23299. PMID: 30990965.