Cat. #153872
pROSA-GUIDE
Cat. #: 153872
Sub-type: Mammalian Expression, Lentiviral, CRISPR
Availability: 3-4 weeks
Target: Gt(ROSA)26Sor; ROSA26
Bacterial Resistance: Ampicillin
Selectable Markers: Puromycin
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Dr Andrew Stephens ; Amy Wilson
Institute: Hudson Institute Of Medical Research
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: pROSA-GUIDE
- Research fields: Cell biology;Genetics
- Tool sub type: Mammalian Expression, Lentiviral, CRISPR
- Backbone size without insert: 11530
- Bacterial resistance: Ampicillin
- Selectable markers: Puromycin
- Description: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5ÄËĂÂĂÂ cloning site: BsmbI 3ÄËĂÂĂÂ cloning site: BsmbI 5ÄËĂÂĂÂ sequencing primer: U6 Forward
- Recommended controls: -
Target Details
- Target: Gt(ROSA)26Sor; ROSA26
Application Details
- Application notes: Validated gRNA targeting ROSA26 locus in mouse cells. Use in combination with pROSA-puro-GFP CRISPR or pROSA-puro-iRFP720 CRISPR to integrate any gene of interest at ROSA26 locus in mouse cells. gRNA in plentiCRISPRv1 backbone. gRNA sequence based on Quadros et al 2015 PMID: 25834785. Species: Mus musculus (house mouse) Cloning method: Restriction Enzyme 5’ cloning site: BsmbI 3’ cloning site: BsmbI 5’ sequencing primer: U6 Forward
