Dicer activation reporter plasmid (DARA plasmid)

Dicer; miRNA; miRNA biogenesis; reporter assay; high-throughput screening

Contributors

Inventor Institute
Andrii Domanskyi, Piotr Chmielarz, Ilmari Parkkinen University of Helsinki
Cat. #: 161705
Research Fields: Cell biology
Application: High throughput screening of chemical compound libraries
Total vector size: 9990 bp
Backbone size without insert: 4329 bp
Bacterial resistance: Ampicillin
Selectable markers: Hygromycin
Primary citation: N/A
Alternate name: pTO-miR19b-tdTom-19b_bind-WPRE-EGFP Dicer-activating reporter assay (DARA) plasmid
Product description: DARA plasmid was created for the Dicer activation reporter assay, which is used to screen compounds for miRNA biogenesis activation. Dicer has been implicated in certain cancers, ALS and Parkinsons Disease. The DARA plasmid was designed to express three main elements: 1) a primary fluorescent reporter protein, a tandem dimer Tomato (tdTomato) red fluorescent protein, which additionally has a nuclear localization sequence (NLS), 3’ untranslated sequence (3’UTR) containing miRNA miR-19b bin…
Application: High throughput screening of chemical compound libraries
Shipping conditions: +4° C or dry ice
Plasmid amplification details: Stbl3 E.coli
Plasmid copy number: High copy number
References:

N/A

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