Negative control iBody (ATTO488)

iBodies™ are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation.

Contributors

Inventor Institute
Jiri Strohalm ; Karel Ulbich ; Petr Cigler ; Pavel Majer ; Frantisek Sedlak ; Tomas Knedlik ; Jirí Schimer ; Jan Konvalinka ; Pavel Sacha ; Sub Vladlmir ; Jan Tykvart ; Vaclav Navratil Institute of Organic Chemistry and Biochemistry ; Charles University ; Institute of Macromolecular Chemistry
Cat. #: 157770
Tool sub type: Marker
Research Fields: Cancer
Purpose: Marker
Product description: – iBodies™ are capable of replacing antibodies in biomedical applications such as ELISA, flow cytometry, confocal microscopy, immunocytochemistry, Western Blot and immunoprecipitation. – These iBodies™ consist of an N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer decorated with low-molecular-weight compounds that function as targeting ligand (e.g., enzyme inhibitors, receptor ligands), affinity anchor (e.g., biotin) and/or imaging probe (various fluorophores). – These iBodies™ have been used for enzyme inhibition, protein isolation, immobilization, quantification and live cell imaging as well as for classical Western Blot application. The system is remarkably modular and can be used for different biological/biochemistry assays.
Additional notes: HPMA conjugate characteristics: MW: 78 041 g/mol #Biotins/molecule: 8 Fluorochrome name: ATTO488 #Fluorochrome/molecule: 5
Storage conditions: Storage at -20° C; solubility H2O; stable at RT, 4° C and -80° C
Shipping conditions: Dry Ice
References:

Ĺ imon et al. 2018. ACS Chem Biol. 13(12):3333-3342. PMID: 30489064.

Dvořáková et al. 2017. J Med Chem. 60(20):8385-8393. PMID: 28953383.

Ĺ ĂĄcha et al. 2016. Angew Chem Int Ed Engl. 55(7):2356-60. PMID: 26749427.

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