#160872

PNPLA1-flox mouse

Cat. #160872

PNPLA1-flox mouse

Cat. #: 160872

Sub-type: Mouse

Availability: 6-8 weeks

Model: Conditional KO

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Contributor

Inventor: Franz Radner

Institute: University of Graz

Tool Details
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: PNPLA1-flox mouse
  • Alternate name: PNPLA1
  • Research fields: Cell signaling and signal transduction;Metabolism
  • Tool sub type: Mouse
  • Model: Conditional KO
  • Conditional: Yes
  • Conditional description: Targeted conditional ready
  • Description: Useful model for the generation of tissue/cell-specific PNPLA1-deficient mice. Animals might be useful for studies, e.g. on skin development/barrier function or omega-O-acylceramide metabolism, or as model for Autosomal Recessive Congenital Ichthyosis (ARCI).
  • Genetic background: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
  • Phenotype: These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
  • Zygosity: Homozygous
  • Strain: C57BL/6J
  • Production details: A floxed neomycin resistance cassette was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette and left Pnpla1 exon 1 floxed. Further details are available upon request.
  • Breeding information: Good breeder. Homozygous mice may be bred together to maintain a live colony,.

Target Details

  • Target: Patatin-like phospholipase domain containing 1, ichthyosis, ARCI

Related Tools

  • Related tools: PNPLA1 Knockout mouse

References

  • Grond et al. 2017. J Invest Dermatol. 137(2):394-402. PMID: 27751867.