PNPLA1-flox mouse

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Tool name: PNPLA1-flox mouse
• Alternate name: PNPLA1
• Research fields: Cell signaling and signal transduction;Metabolism
• Tool sub type: Mouse
• Model: Conditional KO
• Conditional: Yes
• Conditional description: Targeted conditional ready
• Description: Useful model for the generation of tissue/cell-specific PNPLA1-deficient mice. Animals might be useful for studies, e.g. on skin development/barrier function or omega-O-acylceramide metabolism, or as model for Autosomal Recessive Congenital Ichthyosis (ARCI).
• Genetic background: These mice were generated using HM1-ES cells. ES cell clones were injected into C57BL/6J blastocysts to enable coat colour selection of the chimeras. Mice were subsequently backcrossed to C57BL/6J (≥ 10 generations). When kept as a homozygous colony, mice are regularly backcrossed to C57BL/6J to avoid generation of subpopulations.
• Phenotype: These mice are conditional ready, and phenotypical identical to C57BL/6J mice.
• Zygosity: Homozygous
• Strain: C57BL/6J
• Production details: A floxed neomycin resistance cassette was inserted upstream of exon 1. An additional loxP site was inserted downstream of exon 1. Cre-mediated recombination removed the selection cassette and left Pnpla1 exon 1 floxed. Further details are available upon request.
• Breeding information: Good breeder. Homozygous mice may be bred together to maintain a live colony,.

Target Details

• Target: Patatin-like phospholipase domain containing 1, ichthyosis, ARCI

Related Tools

• Related tools: PNPLA1 Knockout mouse

References

• Grond et al. 2017. J Invest Dermatol. 137(2):394-402. PMID: 27751867.