FGFR2b Mouse

Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.

Contributors

Inventor Institute
Clive Dickson Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151695
Research Fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)
disease: Gastric
Zygosity: Homozygous
Product description: Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.
Conditional: Yes
Genetic background: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
Production details: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
Shipping conditions: Embryo/Spermatoza- Dry Ice
References:

Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.

The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.

De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.

An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.

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