Cat. #151695
FGFR2b Mouse
Cat. #: 151695
Sub-type: Mouse
Availability: 6-8 weeks
Disease: Gastric
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Clive Dickson
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Tool name: FGFR2b Mouse
- Research fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
- Tool sub type: Mouse
- Disease: Gastric
- Conditional: Yes
- Description: Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.
- Genetic background: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
- Zygosity: Homozygous
- Production details: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
Handling
- Shipping conditions: Embryo/Spermatoza- Dry Ice
Target Details
- Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)
Related Tools
- Related tools: FGFR2III Floxed Mouse
References
- Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
- The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
- De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
- An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.
