#151695

FGFR2b Mouse

Cat. #151695

FGFR2b Mouse

Cat. #: 151695

Sub-type: Mouse

Availability: 6-8 weeks

Disease: Gastric

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Clive Dickson

Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details
Handling
Target Details
Related Tools
References

Tool Details

*FOR RESEARCH USE ONLY

  • Tool name: FGFR2b Mouse
  • Research fields: Cancer;Cell signaling and signal transduction;Genetics;Immunology
  • Tool sub type: Mouse
  • Disease: Gastric
  • Conditional: Yes
  • Description: Knockout of FGFR2 IIIb isoform; in vivo study of FGFR2 IIIb isoform knockout.
  • Genetic background: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.
  • Zygosity: Homozygous
  • Production details: An Fgfr2 targeting vector, containing a loxP-flanked IIIb exon and a loxP-flanked resistance cassette, was transfected into 129 ES cells. Properly targeted ES cells containing a homologous recombination event were selected, and transfected with a Cre expression vector to excise the resistance cassette. Targeted ES cells were injected into C57BL/6 blastocysts. Chimeric offspring were mated to C57BL/6 mice to generate mice heterozygous for the floxed Fgfr2 allele. Heterozygous mice were interbred to generate mice homozygous for the floxed Fgfr2 allele.

Handling

  • Shipping conditions: Embryo/Spermatoza- Dry Ice

Target Details

  • Target: Fibroblast growth factor receptor 2 isoform IIIb (FGFR2IIIb)

Related Tools

  • Related tools: FGFR2III Floxed Mouse

References

  • Grose et al. 2007. EMBO J. 26(5):1268-78. PMID: 17304214.
  • The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development.
  • De Moerlooze et al. 2000. Development. 127(3):483-92. PMID: 10631169.
  • An important role for the IIIb isoform of fibroblast growth factor receptor 2 (FGFR2) in mesenchymal-epithelial signalling during mouse organogenesis.