SK-MEL-24 Cell Line

SK-MEL-24 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and wildtype N-Ras.

Contributors

Inventor Institute
Lloyd J. Old, Toshitada Takahashi Memorial Sloan-Kettering Cancer Center (MSK)
Cat. #: 161916
Cancer types: Skin cancer
Organism: Human
Tissue: Skin
Gender: Male
Model: Tumourigenic
Morphology: Stellate
Growth properties: Adherent
Cancer Types In Detail: Melanoma
Primary citation: Carey et al. 1976. Proceedings of the National Academy of Sciences. 73: 3278-3282. PMID: 1067619.
Product description: K-MEL-24 is one of a series of melanoma cell lines established from patient-derived tumour samples. This cell line expresses wildtype B-Raf and wildtype N-Ras.
Gender: Male
Model description: Tumourigenic; forms malignant melanoma
Products or characteristics of interest: Isoenzymes: AK-1, 1; ES-D, 1; G6PD, B; GLO-I, 1-2; Me-2, 2; PGM1, 1; PGM3, 2
Storage conditions: Vapor phase of liquid nitrogen
Shipping conditions: Dry Ice
Growth medium: EMEM supplemented with FBS to a final concentration of 15%
Subculture routine: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking
Temperature: 37° C
Atmosphere: 5% CO2 in air
Storage medium: Complete growth medium supplemented with 5% (v/v) DMSO
Biosafety level: 1
References:

Xing et al. 2012. Oncogene. 31(4):446-457. PMID: 21725359.

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