Cat. #160855
SARS-CoV-2 RBD fragment cell line
Cat. #: 160855
Availability: 10-12 weeks
Organism: Human
Disease: Coronavirus
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Institute: The University of Minnesota, Twin Cities
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: SARS-CoV-2 RBD fragment cell line
- Alternate name: Recombinant SARS-CoV-2 RBD
- Cancers detailed: Antigen;Protein fragment;Receptor binding domain
- Research fields: Biochemistry;Drug development;Immunology;Microbiology
- Parental cell: HEK293F
- Organism: Human
- Donor: To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
- Disease: Coronavirus
- Description: The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.
- Production details: To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
- Recommended controls: No
Target Details
- Target: Antigen, protein fragment, receptor binding domain
Handling
- Growth medium: The cells are cultured in FreeStyle 293 Expression Medium from Thermo Fisher.
References
- Shang et al. 2020. Nature. 581(7807):221-224. PMID: 32225175.
