PEO16 Cell Line

PEO16 cell line: Collected after radiotherapy. Part of the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer).

Contributors

Inventor Institute
Simon Langdon Cancer Research UK Edinburgh Centre
Cat. #: 151676
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Ovary
Gender: Male
Model: Tumour line
Growth properties: Adherent
Alternate name: peo16; PE016; PEO-16
Product description: The PEO16 cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists. PEO16 is an adherent cell line from a poorly differentiated serous adenocarcinoma derived from effusion of peritoneal ascites. PEO16 was collected after radiotherapy. PEO16 is tumourigenic in immunologically-deprived CBA mice. PEO16 exhibits poor growth in semi-solid medium (agar). PEO16 was from the same patient as PEO 14
Gender: Male
CRISPR: Yes
Conditional: Yes
Cellosaurus ID: CVCL_2688
Format: Frozen
Shipping conditions: Dry ice
Growth medium: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37?‚°C. Doubling time approximately 40 hours. RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum
Mycoplasma free: Yes
References:

Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737.

Degos et al. 1979. Gastroenterol Clin Biol. 3(10):735-9. PMID: 316786.

Images

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PEO16 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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