PEO14 Cell Line

The PEO14 cell line is one of nine from the PE ovarian adenocarcinoma panel – derived from 4 patients at varying stages of ovarian cancer

Contributors

Inventor Institute
Simon Langdon Cancer Research UK Edinburgh Centre
Cat. #: 151675
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Cancer types: Ovarian cancer
Organism: Human
Tissue: Ovary
Gender: Male
Model: Tumour line
Growth properties: Adherent
Product description: The PEO14 cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages) which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists. PEO14 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a well differentiated serous adenocarcinoma. PEO14 was collected prior to treatment. PEO14 exhibits poor growth in semi-solid medium (agar). PEO14 is from the same patient as PEO16
Gender: Male
CRISPR: Yes
Conditional: Yes
Cellosaurus ID: CVCL_2687
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum.
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 108 hours.
Mycoplasma free: Yes
References:

Langdon et al. 1990. Br J Cancer. 62(2):213-6. PMID: 2386737.

Oestrogen receptor expression and the effects of oestrogen and tamoxifen on the growth of human ovarian carcinoma cell lines.

Degos et al. 1979. Gastroenterol Clin Biol. 3(10):735-9. PMID: 316786.

[The natural history of gastrointestinal bleeding in patients with alcoholic cirrhosis. Definition of a subgroup of patients with high risk of bleeding recurrence (author's transl)]

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PEO14 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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