PEO23 Cell Line

PEO23 was collected on relapse after cisplatin and chlorambucil treatment. PEO23 exhibits poor growth in semi-solid medium (agar). PEO23 is from the same patient asTO14 cell lines.

Contributors

Inventor Institute
Simon Langdon Cancer Research UK Edinburgh Centre
Cat. #: 151677
Tool sub type: 10XUAS IVS p10
Unit size: 1×10^6 cells / vial
Organism: Human
Tissue: Ovary
Gender: Male
Model: Tumour line
Growth properties: Adherent
Alternate name: peo23; PE023; PEO-23
Product description: The PEO23 cell line is one of nine from the PE ovarian adenocarcinoma panel (derived from 4 patients at varying stages of ovarian cancer, isolated from various malignant sites, and at various treatment stages)which provides a model system for research into the mechanism of oestrogen action on ovarian adenocarcinoma tumour cells, and for the study of efficacy and toxicity of oestrogen protagonists. PEO23 is an adherent cell line derived from a malignant effusion from the peritoneal ascites of a patient with a well differentiated serous adenocarcinoma. PEO23 was collected on relapse after cisplatin and chlorambucil treatment. PEO23 exhibits poor growth in semi-solid medium (agar). PEO23 is from the same patient asTO14 cell lines.
Gender: Male
CRISPR: Yes
Conditional: Yes
Cellosaurus ID: CVCL_2689
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI-1640 + 2mM Glutamine + 2mM Sodium Pyruvate + 10% Foetal Bovine Serum.
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10^4 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 120 hours.
Mycoplasma free: Yes
References:

Langdon et al. 1990. Br J Cancer. 62(2):213-216. PMID: 2386737.

Degos et al. 1979. Gastroenterol Clin Biol. 3(10):735-739. PMID: 316786.

Images

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PEO23 Cell Line. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

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