Melan-pe1 cell line
Organism: Mouse
Tissue: Skin
Disease: Hermansky-Pudlak syndrome
Model: Immortalised non-cancerous cell lines
£575.00
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Contributor
Inventor: Elena Sviderskaya
Institute: St. George's University London
Primary Citation: Theos AC, et al. Mol Biol Cell. 2005 Nov;16(11):5356-72. PMID: 16162818
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: Melan-pe1 cell line
- Cancer: N/A
- Research fields: Tissue-specific biology
- Organism: Mouse
- Gender: Unspecified
- Tissue: Skin
- Disease: Hermansky-Pudlak syndrome
- Morphology: Small, oval-fusiform
- Growth properties: Adherent
- Model: Immortalised non-cancerous cell lines
- Crispr: No
- Conditional: No
- Description: The melan-pe1 line is an immortal melanocyte cell line derived from neonatal Ap3b1pe/Ap3b1pe C57BL/6J mice. These cell lines lack Ap3b1, a subunit of the adapter protein-3 (AP-3) complex. AP-3 is involved in sorting the key melanogenic enzyme tyrosinase from early-endosomes towards melanosomes. Pathogenic variants in the human ortholog of Ap3b1 cause a form of Hermansky-Pudlak syndrome (HPS), HPS-2. HPS is a group of disorders characterised by hypopigmentation, bleeding tendency and other variable symptoms. These cells are a useful model for HPS and for the study of trafficking of cargoes towards melanosomes.
- Application: Melanocyte cell biology
- Biosafety level: BSL1
Applications
- Application: Melanocyte cell biology
Handling
- Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
- Storage conditions: liquid N2
References
- Theos AC, et al. Mol Biol Cell. 2005 Nov
- 16(11):5356-72. PMID: 16162819
