Melan-mu2 cell line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: Melan-mu2 cell line
• Cancer: N/A
• Research fields: Tissue-specific biology
• Organism: Mouse
• Gender: Unspecified
• Tissue: Skin
• Disease: Hermansky-Pudlak syndrome
• Morphology: Small, oval-fusiform
• Growth properties: Adherent
• Model: Immortalised non-cancerous cell lines
• Crispr: No
• Conditional: No
• Description: The melan-mu1, -mu2 and mu-3 cell lines are immortal melanocyte cell lines derived from neonatal muted (Mutedmu/mu) Ink4aArf -/-B6.CHMU/Le mice. These cell lines lack muted, a subunit of the biogenesis of lysosome-related organelles complex 1 (BLOC-1). BLOC-1 is involved in cargo sorting from early-endosomes towards lysosome related organelles, including melanosomes. Pathogenic variants in the human ortholog of muted cause a form of Hermansky-Pudlak syndrome (HPS), HPS-11. HPS is a group of disorders characterised by hypopigmentation, bleeding tendency and other variable symptoms. These cells are hypopigmented despite the expression of tyrosinase. These cells are a useful model for HPS and for the study of trafficking of cargoes towards lysosome-related organelles
• Application: Melanocyte cell biology
• Biosafety level: BSL1

Applications

• Application: Melanocyte cell biology

Handling

• Growth medium: RPMI + FBS (10%) + TPA (200 nM) + cholera toxin (CT) (200 pM)
• Storage conditions: liquid N2

References

• Setty SRG, et al. Mol Biol Cell. 2007 Mar
• 18(3):768-80. PMID: 17182843