MEF ULK1 KO (SV40) Cell Line

This cell line is derived from an embryo with a mixed genetic background (Blk6/129 Agouti) and has been immortalised with SV40 large T-antigen.

Contributors

Inventor Institute
Sharon Tooze Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 152609
Unit size: 1×10^6 cells / vial
Research Fields: Apoptosis and autophagy;Cancer;Cell signaling and signal transduction;Metabolism;Neurobiology
Organism: Mouse
Tissue: Embryo
Model: Knock-Out
Growth properties: Fibroblast
Product description: The MEF ULK1 KO (SV40) cell line provides a tool for the study of Ulk1 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk2 KO cell lines. This cell line is derived from an embryo with a mixed genetic background (Blk6/129 Agouti) and has been immortalised with SV40 large T-antigen.
Conditional: Yes
Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 +/+ and the MEFs that were isolated and cultured were immortalised by SV40 using a standard serial passaging protocol.
Cellosaurus ID: CVCL_5A57
disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
References:

McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.

Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.

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