MCF7A estrogen independent breast cancer cell line panel

These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity

Contributors

Institute
Erasmus University Medical Center (Erasmus MC)
Cat. #: 154646
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Model: Cancer Model
Cancer Types In Detail: Estrogen dependent;Mammary adenocarcinoma
Product description: Endocrine therapy of breast cancer has been applied widely and proven to be effective. However, in many instances endocrine treatments ultimately fail due to the development of an estrogen-independent therapy resistant phenotype. To elucidate the molecular mechanism underlying this endocrine therapy failure, the laboratory of Lambert Dorssers applied different genetic screens to identify the main genes conferring estrogen independence. Out of 15 candidate BCAR genes, several including BCAR3, BCAR4 and AKT1 were shown to directly underlie estrogen independence to MCF7A breast cancer cells. These genes were transfected into the MCF7A breast cancer cell line resulting in a panel of 4 cell lines (Cat No 154636-154638, 154643). These cell lines are a powerful tool for studying the molecular and cellular mechanisms of breast tumour progression, therapy resistance and to test the effectiveness of novel drugs to combat different modes of anti-estrogen insensitivity
Conditional: No
Production details: Full length cDNA of the relevant gene was introduced in the estrogen-dependant MCF7A cell line by transfection with lipofectamine
Parental cell line: MCF7A
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS)
References:

Godinho et al. 2011. J Cell Physiol. 226(7):1741-9. PMID: 21506106.

van Agthoven et al. 2010. Endocr Relat Cancer. 17(1):215-30. PMID: 19966015.

van Agthoven et al. 1998. EMBO J. 17(10):2799-808. PMID: 9582273.

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