Cat. #157971
MCF7 ZsGreen-ZNF703 Lenti-control Cell Line
Cat. #: 157971
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Availability: 8-10 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Tumour line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Carlos Caldas
Institute: University of Cambridge
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: MCF7 ZsGreen-ZNF703 Lenti-control Cell Line
- Research fields: Cancer
- Tool sub type: Continuous
- Parental cell: MCF7
- Organism: Human
- Tissue: Breast
- Disease: Cancer
- Model: Tumour line
- Conditional: No
- Description: The MCF7 breast cancer human cell line infected with a control lentivirus (lenti-control) with no gene of interest. ZNF703 drives the exprsesion of the green fluorescent protein ZsGreen. This cell line serves as the negative control for the the MCF7-ZsGreen-ZNF703 overexpressing cell line (see Related tab).
- Biosafety level: 1
Target Details
- Target: Negative control for Zinc Finger Protein 703 (ZNF703) (UniProt ID: Q9H7S9)
Applications
- Application notes: The pHIV-Zsgreen plasmid was cloned by removing the U6-TATAlox-CMVie-EGFP-TATAlox-WPRE content of the pSICO plasmid (Ventura et al., 2004). ZsGreen is used as a reporter for ZNF703.
Handling
- Format: Frozen
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
- Storage conditions: Liquid Nitrogen
- Mycoplasma free: Yes
Related Tools
- Related tools: MCF7 ZsGreen-ZNF703 Overexpressing Cell Line
References
- Holland et al. 2011. EMBO Mol Med. 3(3):167-80. PMID: 21337521.
