MCF7/TAMR-4 Cell Line

Tool Details

*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)

• Name: MCF7/TAMR-4 Cell Line
• Alternate name: MCF-7/TAMR-4 Cell Line
• Cancer: Breast cancer
• Cancers detailed: Breast cancer;Tamoxifen resistant
• Research fields: Cancer;Drug development
• Tool sub type: Continuous
• Parental cell: MCF7 S0.5
• Organism: Human
• Gender: Female
• Tissue: Breast
• Disease: Cancer
• Model: Tumour line
• Conditional: Yes
• Description: MCF7/TAMR-4 cell line is a stable tamoxifen-resistant subline. This cell line has been established in tissue culture after long term treatment with 1 uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat. This cell line was produced from the parental cell line MCF7/S0.5, as a model cell system to study the effects of tamoxife...
• Application: Investigate signalling pathways involved in tamoxifen resistance
• Production details: The parental cell line for the MCF7/TAMR-4 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-4 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line.
• Biosafety level: 1
• Additional notes: MCF7/TAMR-4 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-4 cells are growth inhibited by the pure antiestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-4 cell, ref 1. Treatment targeting the Aurora kinase A restores sensitivity to tamoxifen treatment. The TAMR lines were established from the MCF7/S0.5 cell line, which was adapted to grow with 0.5% fetal calf serum in phenol red containing DMEM/F12 medium. Treatment wi...
• Recommended controls: MCF7-S0.5 parental line
• Cellosaurus id: CVCL_1D42

Target Details

• Target: Oestrogen receptor

Applications

• Application: Investigate signalling pathways involved in tamoxifen resistance
• Application notes: MCF7/TAMR-4 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-4 cells are growth inhibited by the pure antiestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-4 cell, ref 1. Treatment targeting the Aurora kinase A restores sensitivity to tamoxifen treatment. The TAMR lines were established from the MCF7/S0.5 cell line, which was adapted to grow with 0.5% fetal calf serum in phenol red containing DMEM/F12 medium. Treatment wi...

Handling

• Format: Frozen
• Passage number: Passage 402 (AL3482), 403 (AL2753)
• Growth medium: DMEM:Ham's F-12 phenol red-free medium containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM).
• Temperature: 37° C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: MCF7/S0.5 Cell Line

References

• Joshi et al. 2016. Oncotarget. 7(35):57239-57253. PMID: 27528030.
• Elias et al. 2015. Oncogene. 34(15):1919-1927. PMID: 24882577.
• Thrane et al. 2014. Oncogene. PMID: 25362855.
• Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470.
• Cutrupi et al. 2012. Oncogene. 31(40):4353-4361. PMID: 22249258.
• Millour et al. 2010. Oncogene. 29(20):2983-2995. PMID: 20208560.
• Pancholi et al. 2008. Endocr Relat Cancer. 15(4):985-1002. PMID: 18824559.
• Sarwar et al. 2006. Endocr R...