KPC Cell Line (C57/BL6 genetic background)

A transplantable murine PDAC cell line derived from the KPC model on a C57BL/6 background, used for syngeneic immunocompetent studies of tumour–immune interactions, therapeutic response, invasion and metastasis. Exclusive to CancerTools. Save 30% on this cell line this summer. Use code SUM26CELL at checkout.

Contributors

Inventor Institute
Jennifer Morton Cancer Research UK, Glasgow Beatson Institute
SKU: 153474
Product description: The KPC cell line is a transplantable murine pancreatic ductal adenocarcinoma model on a C57BL/6 background, suitable for syngeneic studies in immunocompetent mice. Derived from the clinically relevant KPC model, it retains key KrasG12D and Trp53R172H driver mutations and supports controlled studies of pancreatic tumour biology, therapeutic response, tumour–immune interactions, invasion and metastasis. Its defined background and transplantable format make it useful for bridging in vitro perturbation studies with in vivo validation.
Alternate name: PDAC cell line
Gender: Male
Conditional: No
Production details: These cells are from the KPC mouse which has been backcrossed to F10 in C57/BL6. KPC Subtype: C57Bl6/J. Isolation of the cells: For each cell line, a 5mm diameter piece of tissue was harvested from solid PDAC arising in a C57BL/6 KPC mouse into DMEM. The tissue was chopped into a fine paste, resuspended in DMEM + L-Glut, Pen-Strep and 20% FBS, and plated out in a 25cm flask. The medium was changed after 2 days. Once confluent the cells were passaged as normal and grown in DMEM + L-Glut, Pen-Strep and 10% FBS in normoxic conditions at 37C in a humidified incubator
Disease: Cancer
Cat. #: 153474
Provenance and Ownership: The KPC Cell Line (C57/BL6 genetic background) is owned by Cancer Research UK. CancerTools supplies the KPC Cell Line (C57/BL6 genetic background) under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing KPC Cell Line (C57/BL6 genetic background) directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Pancreatic cancer
Organism: Mouse
Gender: Male
Model: Transgenic
Growth properties: Loosely adherent
Primary citation: Hingorani et al. 2005. Cancer Cell. 7(5):469-83. PMID: 15894267
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: High-glucose DMEM (4.5 g/L) containing 10% foetal bovine serum and 2 mM l-glutamine
Subculture routine: Cells are loosely adherent but will need trypsinising. High-glucose DMEM (4.5 g/L) containing 10% foetal bovine serum and 2 mM l-glutamine with 5% CO2 and air. A 1:2 split can be made every two days which is relatively standard. Incubated as standard
Temperature: 37 ° C
Atmosphere: CO2
Mycoplasma free: Yes
Biosafety level: 1
References:

Sebastiano et al. 2020. Sci. Adv. 6. PMID: 33127675

Li et al. 2014. Gastroenterology. 146(5):1386-96.e1-17. PMID: 24462734

Hingorani et al. 2005. Cancer Cell. 7(5):469-83. PMID: 15894267

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KPC Cell Line (C57/BL6 genetic background) cell sheet monolayer

Citations And Reviews

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