#151710

hTERT-RPE1 mRuby-APC15 Cell Line

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Cat. #151710

hTERT-RPE1 mRuby-APC15 Cell Line

Cat. #: 151710

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Eye

Model: Transgenic

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Jonathon Pines

Institute: University of Cambridge

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: hTERT-RPE1 mRuby-APC15 Cell Line
  • Research fields: Cell biology
  • Parental cell: hTERT-RPE1
  • Organism: Human
  • Tissue: Eye
  • Model: Transgenic
  • Conditional: Yes
  • Conditional description: Tetracycline inducible
  • Description: The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line. APC15 is a component of the human cell cycle Anaphase promoting complex/cyclosome (APC/C). It is required for progression from metaphase during cell cycle. The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to Schizosaccharomyces pombe APC15 (PM gene ID: 2540655), and Schizosaccharomyces cerevisiae Mnd2 (PM gene ID: 854843).
  • Production details: APC15 was amplified by PCR from a full length cDNA clone (ORFeome clone 100066673). Tetracycline inducible cell lines expressing APC15 IRES2 mRuby were generated using the FLIP in system and a modified pCDNA5/FRT/TO vector. The parental tetracycline inducible RPE cell line was created by random integration of an FRT site and a Tet repressor gene into hTERT RPE1 cells followed by selection of a clone that showed high levels of gene expression repression in the absence of tetracycline.
  • Cellosaurus id: CVCL_5F68

Target Details

  • Target: APC15

Handling

  • Format: Frozen
  • Growth medium: F12/DMEM (1:1) media supplemented with 10% FCS, sodium bicarbonate, glutamate and penicillin/streptavidin.
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice

References

  • Mansfield et al. 2011. Nat Cell Biol. 13:1234-43. PMID: 21926987