hTERT-RPE1 mRuby-APC15 Cell Line

The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line.

Contributors

Inventor Institute
Jonathon Pines University of Cambridge
Cat. #: 151710
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology
Organism: Human
Tissue: Eye
Model: Transgenic
Product description: The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line. APC15 is a component of the human cell cycle Anaphase promoting complex/cyclosome (APC/C). It is required for progression from metaphase during cell cycle. The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to Schizosaccharomyces pombe APC15 (PM gene ID: 2540655), and Schizosaccharomyces cerevisiae Mnd2 (PM gene ID: 854843).
Conditional: Yes
Conditional description: Tetracycline inducible
Production details: APC15 was amplified by PCR from a full length cDNA clone (ORFeome clone 100066673). Tetracycline inducible cell lines expressing APC15 IRES2 mRuby were generated using the FLIP in system and a modified pCDNA5/FRT/TO vector. The parental tetracycline inducible RPE cell line was created by random integration of an FRT site and a Tet repressor gene into hTERT RPE1 cells followed by selection of a clone that showed high levels of gene expression repression in the absence of tetracycline.
Cellosaurus ID: CVCL_5F68
Parental cell line: hTERT-RPE1
Format: Frozen
Shipping conditions: Dry ice
Growth medium: F12/DMEM (1:1) media supplemented with 10% FCS, sodium bicarbonate, glutamate and penicillin/streptavidin.
References:

Mansfield et al. 2011. Nat Cell Biol. 13:1234-43. PMID: 21926987

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