#154463

HPV-16-HCK cell line

Cat. #154463

HPV-16-HCK cell line

Cat. #: 154463

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Cervix

Disease: Cancer

Model: Immortalised Line

£430.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Inventor: Aloysius Klingelhutz

Institute: The University of Iowa

Tool Details
Target Details
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HPV-16-HCK cell line
  • Tool sub type: Continuous
  • Parental cell: Human Cervical Keratinocytes
  • Organism: Human
  • Tissue: Cervix
  • Disease: Cancer
  • Growth properties: The HPV-16-containing clones became immortal without a crisis and, at later passage, exhibited elevated levels of telomerase and higher levels of hTERT without any apparent increase in HPV-16 copy number, E6 transcript levels, or ability to degrade p53.
  • Model: Immortalised Line
  • Conditional: No
  • Description: Contains full length HPV16 in episomal form
  • Production details: The 7905 base pair (bp) clone pEFHPV-16W12E (gift from Dr. Paul F. Lambert, University of Wisconsin Medical School, Madison, WI) derived from an HPV-positive patient was utilized as the HPV-16 genome for our replication assays (Flores et al., 1999). One hundred micrograms of HPV-16 genomes was digested overnight in a 750- l reaction from the pUC19 vector using the restriction enzyme BamHI followed by heat inactivation of the enzyme. The entire digested DNA was then re-ligated in a large volum...
  • Biosafety level: 1

Target Details

  • Target: Used as an immortal adult human cervical keratinocyte line for pathogenesis and inflammation studies

Handling

  • Format: Frozen
  • Growth medium: G418 selection (Have with and without feeders)
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Sprague et al. 2002. Virology. 301(2):247-54. PMID: 12359427.