HPV-16-HCK cell line

Contains full length HPV16 in episomal form

Contributors

Inventor Institute
Aloysius Klingelhutz The University of Iowa
Cat. #: 154463
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cancer
Organism: Human
Tissue: Cervix
Model: Immortalised Line
Growth properties: The HPV-16-containing clones became immortal without a crisis and, at later passage, exhibited elevated levels of telomerase and higher levels of hTERT without any apparent increase in HPV-16 copy number, E6 transcript levels, or ability to degrade p53.
Product description: Contains full length HPV16 in episomal form
Conditional: No
Production details: The 7905 base pair (bp) clone pEFHPV-16W12E (gift from Dr. Paul F. Lambert, University of Wisconsin Medical School, Madison, WI) derived from an HPV-positive patient was utilized as the HPV-16 genome for our replication assays (Flores et al., 1999). One hundred micrograms of HPV-16 genomes was digested overnight in a 750- l reaction from the pUC19 vector using the restriction enzyme BamHI followed by heat inactivation of the enzyme. The entire digested DNA was then re-ligated in a large volum…
Parental cell line: Human Cervical Keratinocytes
disease: Cancer
Format: Frozen
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry ice
Growth medium: G418 selection (Have with and without feeders)
Mycoplasma free: Yes
Biosafety level: 1
References:

Sprague et al. 2002. Virology. 301(2):247-54. PMID: 12359427.

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