Cat. #157701
HEK-ADAM10 CRISPR cell line
Cat. #: 157701
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Kidney
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Michael Tomlinson
Institute: University of Birmingham
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: HEK-ADAM10 CRISPR cell line
- Alternate name: A Disintegrin And Metalloproteinase domain-containing protein 1, CRISPR-Cas9
- Research fields: Cancer;Neurobiology
- Tool sub type: Continuous
- Parental cell: HEK 293T
- Organism: Human
- Tissue: Kidney
- Disease: Cancer
- Description: A disintegrin and metalloprotease 10 (ADAM10) is essential for embryonic development and impacts on diseases such as cancer, Alzheimer?Âs and inflammatory diseases. ADAM10 is a ?Âmolecular scissor? that proteolytically cleaves the extracellular region from over 100 substrates, including Notch, amyloid precursor protein, cadherins, growth factors and chemokines.CRISPR edited ADAM 10 HEK-293T cells.Generation of CRISPR/Cas9-knockout cell line:A guide RNA sequences was selected for human ADAM10, and the following primer pairs were used to encode these sequences: ADAM10 guide 2 (5?Â-CACCGATACCTCTCATATTTACAC-3? and 5?Â-AAACGTGTAAATATGAGAGGTATC-3?Â)
Target Details
- Target: ADAM10
Handling
- Format: Frozen
- Growth medium: Cancer Research Technology Limited (trading research tools as Ximbio) has been granted a non-exclusive license to the CRISPR-Cas9 technology by ERS Genomics Ltd under the patent rights listed here.This license from ERS Genomics Ltd allows Ximbio to develop and commercialise CRISPR-Cas9 modified cell lines for research use only. Ximbio can provide these modified CRISPR-Cas9 ...
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Tetraspanin Tspan15 is an essential subunit of an ADAM10 scissor complex
- Brummer et al. 2018. FASEB J. 32(7):3560-3573. PMID: 29430990.
