#154153

HEK 293 cell line (genotyped)

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Cat. #154153

HEK 293 cell line (genotyped)

Cat. #: 154153

Sub-type: Continuous

Unit size: 1x10^6 cells / vial

Availability: 3-4 weeks

Organism: Human

Tissue: Kidney

Model: Immortalised Line

£575.00

This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.

Contributor

Institute: Vlaams Instituut voor Biotechnologie (VIB)

Tool Details
Applications
Handling
References

Tool Details

*FOR RESEARCH USE ONLY

  • Name: HEK 293 cell line (genotyped)
  • Research fields: Cancer
  • Tool sub type: Continuous
  • Parental cell: Human embryonic kidney cells
  • Organism: Human
  • Tissue: Kidney
  • Model: Immortalised Line
  • Conditional: No
  • Description: The human embryonic kidney (HEK) 293 cell line and its derivatives are used in experiments ranging from signal transduction and protein interaction studies over viral packaging to rapid small-scale protein expression and biopharmaceutical production. The original 293 cells were derived in 1973 from the kidney of an aborted human embryo of unknown parenthood by transformation with sheared Adenovirus 5 DNA. The human embryonic kidney cells at first seemed recalcitrant to transformation. After m...
  • Production details: HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA
  • Biosafety level: 1
  • Cellosaurus id: CVCL_0045

Applications

  • Application notes: These HEK 293 have been whole-genome re-sequenced at passage number 35. The article by Lin et al al, allows user friendly visualisation of the genome data. The genome structure composition is in steady state when standard cell culturing conditions are used.

Handling

  • Format: Frozen
  • Growth medium: EMEM (EBSS) + 2mM Glutamine + 1% Non-Essential Amino Acids (NEAA) + 10% FCS
  • Unit size: 1x10^6 cells / vial
  • Shipping conditions: Dry ice
  • Storage conditions: Liquid Nitrogen
  • Mycoplasma free: Yes

References

  • Lin et al. 2014. Nat Commun. 5:4767. PMID: 25182477.
  • Genome dynamics of the human embryonic kidney 293 lineage in response to cell biology manipulations.
  • Stillman et al. 1985. Mol Cell Biol. 5(8):2051-60. PMID: 3018548.
  • Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.