Cat. #153246
fR2 Cell Line
Cat. #: 153246
Unit size: 1x10^6 cells / vial
Availability: 10-12 weeks
Organism: Human
Tissue: Breast
Disease: Cancer
Model: Immortalised Line
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Joyce Taylor-Papadimitriou ; Sidney Chang
Institute: Cancer Research UK, Lincoln's Inn Fields Institute
Primary Citation: Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.
Tool Details
*FOR RESEARCH USE ONLY (for other uses, please contact the licensing team)
- Name: fR2 Cell Line
- Cancer: Breast cancer
- Cancers detailed: Breast cancer
- Research fields: Cancer;Drug development
- Organism: Human
- Gender: Female
- Tissue: Breast
- Disease: Cancer
- Growth properties: Adherent
- Model: Immortalised Line
- Conditional: No
- Description: The fR2 Cell Line was established by infecting milk cultures with with simian vacuolating virus 40 (SV40). fR2 cells express Muc-1 (also known as HMFG-1 antigen), which is a mucin-like component of human milk fat globule membranes. fR2 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice. This cell line is positive for keratin 8 and 18, but negative for keratins 4, 6, 7, 10, 13, 14, 16, 17 and 19. Although fR2 is morphologically different from fR5 cell line, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. This cell line could be useful for studying the relationship between transformation and differentiation in human mammary epithelial cells.
- Application: Investigating relationship between transformation and differentiation
- Production details: The breast epithelial cell line fR2 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40.
- Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20
- Cellosaurus id: CVCL_2444
Applications
- Application: Investigating relationship between transformation and differentiation
- Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 12,13 D13S317: 12 D16S539: 11,13 D5S818: 12,13 D7S820: 8,12 THO1: 9.3 TPOX: 8 vWA: 20
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:10 i.e. seeding 1 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37° C. Culture Medium: RPMI 1640 + 2mM Glutamine + 10ug/ml insulin + 5ug/ml hydrocortisone + 10% FBS.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
References
- Chang et al. 1982. Cancer Res. 42(5):2040-2053. PMID: 6279290.
